Karthikeyan Jothi

• Performed various Illumina Array Assay
• Infinium Global Screening Array
• Infinium Global Diversity Array
• Infinium MethylationEPIC
• Infinium Global Screening Array with Cytogenetics
• HumanKaryomap - Karyomapping Microarray
• Bisulfite conversion of DNA for Illumina Methylation Array
• Data processing with Genome Studio, sharing data and outcomes with the Bio Informatics team
• Creating and presenting monthly work report to Quality Assurance Department (QAD) on behalf of team.
• Send an email to the Illumina team with any troubleshooting regarding the Array and using it.
• Responsible for updating and creating various SOPs and other organisational charts for the smooth flow of processes.
• Past Job Responsibilities:
• Performed manual and automated DNA/cfDNA/RNA extraction from biological samples.
• Performed the quality check of DNA/cfDNA/RNA samples which includes Spectrophotometric, fluorimetric estimation and Agarose gel electrophoresis system.
• Histopathology Section cutting for DNA and RNA extraction for genetic testing
• Performing various Molecular Genetic Tests:
• Real Time Pcr tests - JAK2 Mutation , BCR-ABL, HLA- B27,KRAS MUTATION,EGFR MUTATION, FLT3, MSI, NRAS, BRAF, PIK3C
• Glioma panel 1p19q codeletion, Oligodendroglioma, MGMT, BRCA 1 & BRCA 2 Deletions and Duplications, MPN, HRD, HRR, TMB.
• DNA/RNA library preparation for sequencing
• Performed RT-PCR of Covid samples.
• Responsible for maintaining the logs pertaining to cleaning and Instruments and Temperature in BSL-2 Lab.
• Recorded, reported and posted test results using LIMS
• Allotment of samples to team in accordance to turnaround time

• Analyzed bodily fluids with laboratory equipment and detected anomalies related to diseased states or acute injuries.
• Conduct lab tests in the areas of Hematology, Urinalysis,Biochemistry ,Serology and Microbiology and assisted in grossing of Histopathology samples, Tissue Processing and Section cutting.
• Prepare chemical solutions, reagents and specimens for microscopic examination
• Recorded,reported and posted test results using LIMS

Instrument familiarized: BD FACS CantoII, Coulter LH750, Coulter LH780, Sysmex-XN1000,

• Flowcytometry- CD34 Count, Differentiate from Myeloid and Lymphoid series based on adding antibodies like Cluster of Differentiation(45,34,FITC,19,20,10,33,16,3,7,IREM,14,38,123,117 etc.)
• Wright-Giemsa Stain(Bone Marrow)
• Special Staining (MPO, Sudan Black, Pearls Iron Staining for Bone Marrow)
• Estimation of Leukocyte Alkaline Phosphatase, Double Esterase & Hemoglobin parameters
• Blood Picture Studies, ESR, TC, DC, RBC, Platelet, Retics
• Estimation of Red cell indices: AEC, MCH, MCV, MCHC, HB%
• Microscopic detection of Malarial Parasite & Microfilaria (QBC Method)
• Studies of cavity fluids (CSF, Pleural, Peritoneal).