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S Sugumar
Bioprocess Specialist
Chennai
Summary
Bioprocess Specialist with expertise in Microbial fermentation, Mammalian Cell culture and Downstream processing.
Organized and dependable candidate successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals.
Overview
22
22
years of professional experience
1
1
Language
Work History
Senior Research Team Leader - Biotechnology Pilot plant
Orchid Pharma Ltd.
Alathur, Chennai
01.2022 - Current
- Coordinated in the setup of Fermentation Lab with skid of four numbers of 10L fermenters, and establishing Pilot scale Fermentation setup of 19L, 50L, and 200L and Downstream processing Lab.
- Supervised a team of 8 members in Fungal Fermentation.
- Undergone month long fermentation trial in South Korea on Technology transfer.
- Process Development in 10L Lab Scale Fermenters.
- Process Scale up in 19L, 50L and 200L Fermenters.
- Downstream processing involving Microfiltration, Ultrafiltration, Column Chromatography, Product Isolation, Nanofiltration, and Crystallization.
Assistant Manager, Production division
Biovet Pvt. Ltd.
Malur
03.2022 - 10.2022
- Involved in Media preparation, Cell-culture, Virus Infection and Inactivation, Antigen Concentration for FMD Vaccine, major product of Biovet.
- Involved in Production, Inactivation, and Antigen Concentration for Bacterial Vaccine.
- Coordinated in Formulation and Filling of Bacterial Vaccine and FMD Vaccine.
- Coordinated OQ for 240L Fermenter installed in new facility.
- Involved in Documentation and Material Management.
Biotechnologist I - Biotechnology division
ASN Fuels Pvt. Ltd.
Tirupati
01.2020 - 01.2022
- Optimized the Pre-Treatment Method for Feedstocks like Corncobs and Bagasse.
- Saccharification process optimization in Tube Manifold Reactor for the production of Glucose being carried out.
- Optimization of the Yeast Fermentation process in Loop Fermenter for the production of Ethanol being carried out.
- Coordinated Batch Planning, Operation, Analysis and Documentation.
- Involved in Manpower and Material Management.
- Saccharification Batch Scaled-up from 70L to 600L Capacity in Tube Manifold Reactor.
Research Scientist II - Biotechnology division
Orchid Pharma Ltd.
Chennai
01.2010 - 01.2019
- Supervised a team of 12 members in Fungal, Bacterial Fermentation and Downstream processing.
- Optimized the fermentation process for the production of SAMe using the recombinant Pichia pastoris and improved yield by 30 percent.
- Optimized the fermentation process for the production of Enzymes in Cephalosporin pathway using recombinant E.Coli strains and improved yield by 40 percent.
- Process Development and Optimization for both Upstream and Downstream.
- Scale-up and Technology Transfer carried out from 19L to 200L Fermentor.
- Authored and reviewed Batch Records, SOPs, and Protocols.
- Coordinated with QA in Closure of Batch Records and Process related deviations.
- Trained on Basic Cell culture handling methods, Revival of Frozen Cell lines.
- Root Cause Analysis using tools like Fish Bone Diagram, 5 Whys.
- Inventory management for Media Chemicals, Spares and Equipments Service.
Research Scientist I - Biotechnology division
Orchid Chemicals and Pharmaceuticals Ltd.
Chennai
01.2008 - 01.2010
- Coordinated in the setup of 200L Pilot scale Fermentation and Downstream processing Lab.
- Supervised a team of 8 members in Fungal and Bacterial Fermentation.
- Process Development in 7L, 16L and 19L Lab Scale Fermenters.
- Process Scale up in 50L and 200L Fermenters.
- Downstream processing involving Protein Isolation, Quantification, and Enzyme Assays.
- Trained and Motivated Team members towards fermentation and downstream process.
- Participant of Internal Safety Committees, PPE and Contractors Safety Management.
Research Associate - Biotechnology division
Orchid Chemicals and Pharmaceuticals Ltd.
Chennai
01.2007 - 01.2008
- Optimized Production of Cephalosporin using recombinant Penicillium chrysogenum, achieved yield in grams per litre.
- Process Development in 16L and 50L Lab Scale Fermenters.
- Optimized Production of Cephalosporin Pathway enzymes using recombinant E.Coli.
Research Executive - Biotechnology division
Orchid Chemicals and Pharmaceuticals Ltd.
Chennai
01.2004 - 01.2007
- Transformation / Modification of recombinant Penicillium to produce Cephalosporin.
- Method development for Screening and Bioassay.
- Optimized Conditions for Production of Cephalosporin using recombinant Penicillium strains developed in house.
Education
M.Tech - Biotechnology
A.C. Tech, Anna University
01.2004
B.Tech - Chemical Engineering
A.C. Tech, Anna University
01.2002
Skills
- Bioprocess Engineering – Expertise in Handling Fermenters from Lab scale to Pilot scale (7L to 200L) capacity for Bacterial and Fungal fermentation
- Fermentation of Yeast and recombinant Penicillium chrysogenum, Pichia pastoris and Escherichia coli
- Downstream processing techniques – Dynomill, Homogenization, Filter Press/ Centrifugal Broth separation, Protein Isolation
- Analytical Instrumentation and Concepts - HPLC, SDS PAGE, YSI multi-parameter analyser, Protein Quantification and Enzyme Assays
- Cell Culture techniques – Working with Biosafety Cabinets and Incubator, Dulbecco's modified eagle medium (DMEM) media preparation, Clean Cell Culture techniques, Reviving of frozen cell lines, Subculture of adherent cell lines, Cell quantification using haemocytometer (Trypan blue), Cryopreservation of cell lines
- Preparation of Batch Records, SOPs and Protocols
- Root Cause Analysis using tools like Fish bone diagram, 5 Whys
Accomplishments
- Presented Paper titled “Fermentation kinetics of glucose solution in a LOOP Reactor to yield Ethanol” in Symposium on “Challenges in the production of 2G Bioethanol” organised by Dept. Of Chemical Engineering, IIT-Tirupati.
- Coordinated Setup of 600L Pilot scale Saccharification Tube Manifold reactor and 140L Tube Manifold Fermenter
- Coordinated Setup of 200L Pilot scale Fermentation and Downstream processing Lab.
- Produced and supplied 2000L of Cephalosporin Esterase Enzyme for Factory requirement.
- Transferred 2 Bacterial and 1 Pichia process to manufacturing scale.
PROCESS PATENTS:
- 1. A Bioprocess for the manufacture of semi synthetic Cephalosporins, Orchid Chemicals and Pharmaceuticals Ltd, India, 1638/CHE/05, 9.11.2005
- 2. Mutated Cephalosporin hydroxylase and its application in deacetyl Cephalosporanic acid synthesis. Orchid Chemicals and Pharmaceuticals Ltd, WO2013105030. 18/7/2013.
PERSONAL DETAILS:
- Date of Birth: 9th March 1981
- Nationality: Indian
- Gender / Marital Status: Male/ Married
- Vaccination Status: Vaccinated (2 doses of Covaxin)
Software
Timeline
Assistant Manager, Production division
Biovet Pvt. Ltd.
03.2022 - 10.2022
Senior Research Team Leader - Biotechnology Pilot plant
Orchid Pharma Ltd.
01.2022 - Current
Biotechnologist I - Biotechnology division
ASN Fuels Pvt. Ltd.
01.2020 - 01.2022
Research Scientist II - Biotechnology division
Orchid Pharma Ltd.
01.2010 - 01.2019
Research Scientist I - Biotechnology division
Orchid Chemicals and Pharmaceuticals Ltd.
01.2008 - 01.2010
Research Associate - Biotechnology division
Orchid Chemicals and Pharmaceuticals Ltd.
01.2007 - 01.2008
Research Executive - Biotechnology division
Orchid Chemicals and Pharmaceuticals Ltd.
01.2004 - 01.2007
B.Tech - Chemical Engineering
A.C. Tech, Anna University
M.Tech - Biotechnology
A.C. Tech, Anna University