Summary
Overview
Work History
Education
Skills
AWARDS & RECOGNITION
SELECTED PUBLICATIONS
TEACHING & TRAINING EXPERIENCE
UNIVERSITY & PROFESSIONAL SERVICE
Timeline
Generic

Sanjay K. Singh

Johnson City,TN

Summary

Versatile microbiologist and molecular biologist with extensive experience investigating host–pathogen interactions, inflammatory disease models, and structure–function relationships of C-reactive protein (CRP). Over 20 years of hands-on expertise in microbial culture, animal infection models, biochemical assays, and protein engineering across multiple academic research environments. Proven ability to design experiments, execute complex studies, analyze data, and maintain high standards of laboratory accuracy, sterility, and safety. Highly skilled in teaching, mentoring, and supervising laboratory personnel, including undergraduate, graduate, and medical students. Strong record of scientific productivity, with numerous peer-reviewed publications and contributions to NIH-funded research programs. Recognized for excellence in teaching and student engagement, including being selected as 2025 Professor of the Year. Brings deep experience in aseptic technique, microbial manipulation, biochemical assays, molecular biology methods, and GLP-aligned lab organization—well-suited for cross-functional R&D teams working in microbiology, assay development, and experimental research.

Overview

32
32
years of professional experience

Work History

Research Associate Professor

East Tennessee State University
01.2020 - Current
  • Lead microbiology and immunology research projects investigating CRP structure–function relationships.
  • Conduct microbial infection experiments in mouse models (pneumococcal infection, inflammatory arthritis).
  • Perform microbial assays, cytokine analysis, and immune modulation studies.
  • Design and execute experiments, analyze data, and maintain accurate lab documentation.
  • Train and supervise undergraduate, graduate, and medical students in lab techniques, safety, and experimental design.
  • Teach graduate courses including Biochemistry, Cell Biology and Microbiology.
  • Maintain laboratory readiness, compliance, and sterile workflows for ongoing research.

Research Assistant Professor

East Tennessee State University
01.2015 - 01.2020
  • Investigated CRP function in atherosclerosis, pneumococcal infection, and inflammatory arthritis.
  • Led in vivo studies involving bacterial challenges and immune profiling.
  • Mentored graduate students and participated in the development of NIH-funded research aims.

Research Assistant Professor

Louisiana State University
01.2014 - 01.2015
  • Conducted research on inflammasomes in bacterial infection.
  • Performed microbial and molecular biology assays consistent with host-response investigations.

T32 Research Fellow

University of Louisville
01.2013 - 01.2014
  • Engineered pancreatic islets using TGF-β constructs for Type I diabetes applications.

Research Associate / Instructor / Postdoctoral Roles

University of Louisville, Ohio State University, ETSU
01.2003 - 01.2013
  • Worked on nanoparticle-based drug delivery, mucosal vaccine development, and CRP biology.
  • Extensive hands-on experience in protein chemistry, molecular cloning, microbial interactions, and immunology.

Lecturer

Kutir Postgraduate College, India
01.1994 - 01.1996

Education

Ph.D. - Zoology

Banaras Hindu University
India
2002

M.Sc. - Biology

Purvanchal University
India
1993

B.Ed. - Education

Purvanchal University
India

B.Sc. - Biology

Purvanchal University
India

Skills

  • Microbiology & Cell-Based Methods
  • Microbial culture, isolation, and enumeration
  • Aseptic/sterile technique (BSL-2)
  • Murine infection models: pneumococcal infection, inflammatory arthritis, atherosclerosis
  • Environmental monitoring & sterility considerations
  • Host–pathogen interaction studies
  • Immune response and cytokine analysis
  • Molecular Biology & Biochemistry
  • Protein engineering, mutagenesis, recombinant expression
  • Protein purification (affinity, ion exchange, gel filtration chromatography)
  • SDS-PAGE, Western blot, immunoassays
  • Enzymatic assays & molecular characterization
  • PCR-based workflows
  • Data & Documentation
  • Experimental design, statistical analysis
  • Manuscript preparation & scientific presentations
  • Laboratory notebook documentation (electronic & paper)
  • GLP/GMP understanding & compliance
  • Instruction & Leadership
  • Classroom instruction, lab course supervision, graduate-level teaching
  • Supervision of technicians, undergraduate students, graduate students, and medical residents
  • Curriculum development and mentoring for research projects

AWARDS & RECOGNITION

  • Early Career Faculty Travel Award, AAI (2015–2020)
  • Professor of the Year, Biomedical Sciences Graduate Program, ETSU (2025)
  • Qualified GATE (Graduate Aptitude Test in Engineering), India

SELECTED PUBLICATIONS

  • Agrawal A., Pathak A., Ngwa D.N., Thirumalai A., Armstrong P.B., Singh S.K. CRP prevents formation of amyloid fibrils. Frontiers in Immunology, 2024.
  • Singh S.K., Prislovsky A., Ngwa D.N., et al. CRP lowers IL-17 and reduces arthritis incidence in mice. Frontiers in Immunology, 2024.
  • Singh S.K., Ngwa D.N., Agrawal A. CRP-based strategy to reduce antibiotic dosing. Frontiers in Immunology, 2021.
  • Singh S.K., Pathak A., Thewke D.P., Agrawal A. Conformationally altered CRP reduces atherosclerosis. Frontiers in Immunology, 2020.
  • Singh S.K., Thirumalai A., Hammond D.J., et al. Functional transformation of CRP by hydrogen peroxide. Journal of Biological Chemistry, 2017.

TEACHING & TRAINING EXPERIENCE

  • Instructor for graduate and undergraduate courses in Biochemistry, Molecular Biology, and Gene Regulation at ETSU.
  • Director and Supervisor of laboratory courses and teaching assistants.
  • Trained 3 technicians, 5 undergraduate students, 8 graduate students, and 8 medical fellows.
  • Proficient with D2L and Panopto teaching platforms.
  • Awarded Professor of the Year (2025).

UNIVERSITY & PROFESSIONAL SERVICE

  • Judge, ETSU Appalachian Student Research Forum
  • Judge, Dr. Jay W. Boland Research Day (2025)
  • Graduate Academic Misconduct / Grade Appeal Committee
  • Reviewer for scientific journals including Frontiers in Immunology, Virulence, PLoS One

Timeline

Research Associate Professor

East Tennessee State University
01.2020 - Current

Research Assistant Professor

East Tennessee State University
01.2015 - 01.2020

Research Assistant Professor

Louisiana State University
01.2014 - 01.2015

T32 Research Fellow

University of Louisville
01.2013 - 01.2014

Research Associate / Instructor / Postdoctoral Roles

University of Louisville, Ohio State University, ETSU
01.2003 - 01.2013

Lecturer

Kutir Postgraduate College, India
01.1994 - 01.1996

Ph.D. - Zoology

Banaras Hindu University

M.Sc. - Biology

Purvanchal University

B.Ed. - Education

Purvanchal University

B.Sc. - Biology

Purvanchal University
Sanjay K. Singh